Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Isolement des cellules mononucléaires Lamina…
JoVE Journal
Immunology and Infection

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunology and Infection
Isolement des cellules mononucléaires Lamina Propria du colon murine à l'aide de la collagène E
 
Click here for the English version

Isolement des cellules mononucléaires Lamina Propria du colon murine à l'aide de la collagène E

Article DOI: 10.3791/59821-v • 09:48 min • September 26th, 2019
September 26th, 2019 •
1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4,5
1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation, University of Miami Miller School of Medicine, 2Batchelor Children's Research Institute, University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology, University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 5Holtz Children's Hospital, University of Miami Miller School of Medicine

Capitoli

Riepilogo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Le but de ce protocole est d'isoler les cellules mononucléaires qui résident dans la lamina propria du côlon par digestion enzymatique du tissu à l'aide de collagène. Ce protocole permet l'isolement efficace des cellules mononucléaires résultant en une suspension de cellules simples qui à son tour peut être utilisé pour l'immunophénotypage robuste.

Tags

Immunologie et infection Numéro 151 Lymphocytes côlon murine lamina propria colite transplantation cytométrie du flux digestion enzymatique inflammation cellules mononucléaires
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter