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在肽膜前体的维西图洛合成中,用于自主囊泡生长
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JoVE Journal Bioingegneria
In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
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在肽膜前体的维西图洛合成中,用于自主囊泡生长

DOI:
10.3791/59831-v

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07:10 min

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June 28, 2019

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, , , , , , ,
1Physics of Synthetic Systems – E14, Physics-Department and ZNN,Technische Universität München, 2Departmant of Chemistry, Center for Integrated Protein Science (CIPSM),Technische Universität München, 3Nanosystems Initiative Munich

Capitoli

  • 00:04Titolo
  • 00:29Vesicle Production Using the Glass Beads Method
  • 02:24Transcription-translation (TX-TL) Reaction
  • 05:07Results: Analysis of Synthesized Peptide Membrane Precursors
  • 06:41Conclusion

Summary

Traduzione automatica

Traduzione automatica

这里介绍了创建能够生长的基于肽的小型单片杆菌囊泡的协议。为了便于膜肽的囊泡生产,这些囊泡配备了转录翻译系统和肽编码质粒。

Tags

Peptide Membrane PrecursorsAutonomous Vesicle GrowthFilm Re-hydrationReaction CompartmentsELP SolutionChloroform MethanolGlass BeadsRotary EvaporatorVesicle FormationPlasmid DNA PurificationPhase SeparationEthanol Precipitation

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