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洗涤剂辅助重组重组果蝇阿拉他素到脂质混合测定脂质体

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Detergent-assisted Reconstitution of Recombinant Drosophila Atlastin into Liposomes for Lipid-mixing Assays

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洗涤剂辅助重组重组果蝇阿拉他素到脂质混合测定脂质体

DOI:

10.3791/59867-v

•

08:43 min

•

July 03, 2019

•

Miguel A. Betancourt-Solis, James A. McNew

¹Department of BioSciences,Rice University

Capitoli

00:04Titolo
00:56Liposome Production by Extrusion Method
02:45Reconstitution by Detergent Assisted Incorporation into Preformed Liposomes
05:10Lipid Mixing Assays
06:16Results: Analysis of Liposomes Reconstituted from Recombinant Drosophila Atlastin
07:58Conclusion

Summary

Traduzione automatica

生物膜融合由专用融合蛋白催化。通过脂质混合测定,可以测量蛋白质的富源性。我们提出了一种纯化重组果蝇蛋白的方法,这是一种蛋白质,可以调节ER的同质融合,将其重组为预制脂质体,并测试融合能力。

Tags

Keywords: Detergent-assisted Reconstitution Recombinant Drosophila Atlastin Liposomes Lipid-mixing Assays FRET-based Lipid-mixing Proteoliposomes Lipid Film Freeze-thaw Extrusion Lipid Concentration

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