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Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
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1Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud,Hospices Civils de Lyon, 2Circulating Cancer (CIRCAN) Program,Hospices Civils de Lyon Cancer Institute, 3University of Lyon, Claude Bernard University,Cancer Research Center of Lyon, 4Biolidics Limited, 5Institut de Pathologie Multisites des HCL-Site Sud,Hospices Civils de Lyon, 6Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital,Hospices Civils de Lyon Cancer Institute, 7EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty,Lyon 1 University
Capitoli
- 00:04Titolo
- 01:11Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples
- 03:10Spiral Microfluidic Device CTC Enrichment
- 04:20Immunofluorescence (IF) Staining
- 06:32IF Image Acquisition and Analysis
- 08:07Results: Representative CTC Characterization and Enumeration
- 09:56Conclusion
The characterization of circulating tumor cells (CTCs) is a popular topic in translational research. This protocol describes a semi-automatic immunofluorescence (IF) assay for PD-L1 characterization and enumeration of CTCs in non-small cell lung cancer (NSCLC) patient samples.
