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Immunologia e infezioni

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调整降解以实现特定和高效的蛋白质消耗

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

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调整降解以实现特定和高效的蛋白质消耗

DOI:

10.3791/59874-v

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05:11 min

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July 20, 2019

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J. David Barrass, Gonzalo I. Mendoza-Ochoa², Isabella E. Maudlin³, Emanuela Sani, Jean D. Beggs

¹Wellcome Centre for Cell Biology, School of Biological Sciences,University of Edinburgh, ²Department of Plant Sciences,University of Cambridge, ³Sir William Dunn School of Pathology,University of Oxford

Capitoli

00:04Titolo
00:36General Procedure for Depletion
03:31Results: Analysis of the Tuned Depletion Rate
04:25Conclusion

Summary

Traduzione automatica

在这里,我们提出一个协议,使用β-est AID系统,有效和具体地耗尽酵母糖精中感兴趣的蛋白质。

Tags

Protein Depletion Essential Protein Protein Function Protein Removal Auxin Beta-estradiol IAA Cell Death Cell Culture Optical Density Sample Collection Centrifugation

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