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1Department of Pharmaceutical Sciences, College of Pharmacy,University of Tennessee Health Science Center, 2Department of Urology,Medical University of South Carolina, 3Department of Urology, College of Medicine,University of Tennessee Health Science Center, 4Department of Pharmacology, College of Medicine,University of Tennessee Health Science Center
Capitoli
- 00:04Introduction
- 01:45Dissection and Enzymatic Dissociation of Mucosa-free DSM Pieces
- 03:28Voltage-step Induced Cation Currents from DSM Cells Using Amphotericin-B Perforated Whole-cell Voltage Patch-clamp Technique
- 05:52Results: Investigation of Ion Channel Properties in Isolated DSM Cells
- 07:07Conclusion
Biz iki adım lı enzimatik prosedür istihdam insan idrar kesesi örnekleri tek taze izole detrusor düz kas hücrelerinin hazırlanması için bir yöntem açıklar. Elde edilen canlı DSM hücreleri fizyolojik ve farmakolojik özellikleri ortaya çıkarmak için açıklanan amphotericin-B patch-clamp elektrofizyolojisi de dahil olmak üzere çeşitli tek hücre teknikleri ile incelenebilir.
