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从小鼠横纹肌瘤分离的原发性肿瘤细胞的肿瘤球衍生和治疗

JoVE Journal
Genetica

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JoVE Journal Genetica

Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas

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从小鼠横纹肌瘤分离的原发性肿瘤细胞的肿瘤球衍生和治疗

DOI:

10.3791/59897-v

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09:21 min

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September 13, 2019

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Francesca Boscolo Sesillo^2,3, Alessandra Sacco

¹Graduate School of Biomedical Sciences,Sanford Burnham Prebys Medical Discovery Institute, ²Development, Aging and Regeneration Program,Sanford Burnham Prebys Medical Discovery Institute, ³Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery,University California San Diego

Capitoli

00:04Titolo
01:44Cell Isolation and Culture
03:46Tumorsphere Derivation
05:02Tumorsphere Preparation for Allograft Transplantation
07:26Results: Tumorsphere Derivation and Transfection
08:46Conclusion

Summary

Traduzione automatica

该协议描述了一种从肿瘤层培养开始分离小鼠横纹肌瘤原发细胞、肿瘤球的形成和治疗以及异体移植的可重复方法。

Tags

Rhabdomyosarcoma Tumorsphere Formation Assay Cell Of Origin Myogenic Markers Tumor-originating Cells Spheroid Structure Drug-screening Cell Isolation Collagenase Digestion Cell Suspension

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