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Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems
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Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

DOI:
10.3791/59903-v

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09:35 min

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September 13, 2019

•

,
1Department of Aquatic Microbial Ecology, Institute of Hydrobiology,Biology Centre CAS

Capitoli

  • 00:04Titolo
  • 01:11Sample Collection and Fixation
  • 01:44Sample Filtration and Enumeration of Bacterial Numbers on the Filters
  • 02:45Determining Protistan Abundance
  • 03:28Estimating Ciliate Grazing Rates
  • 04:33Results: Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems
  • 08:07Conclusion

Summary

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Presented here is a protocol for a single-cell, epifluorescence microscopy-based technique to quantify grazing rates in aquatic predatory eukaryotes with high precision and taxonomic resolution.

Tags

Fluorescently Labeled BacteriaOrganic Carbon FlowAquatic EcosystemsMicrobial Food WebsPredator-prey InteractionsGrazing RatesTrophic InteractionsNutrient TransferSingle-cell AnalysisMicroscopyEpifluorescenceAquatic Microbial Communities

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