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活细胞荧光显微镜研究细菌的亚细胞蛋白定位和细胞形态变化
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
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活细胞荧光显微镜研究细菌的亚细胞蛋白定位和细胞形态变化

DOI:
10.3791/59905-v

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05:57 min

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November 23, 2019

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, ,
1Department of Cell Biology, Microbiology and Molecular Biology,University of South Florida

Capitoli

  • 00:04Titolo
  • 00:48Sample Preparation
  • 01:39Imaging
  • 03:34Deconvolution and Data Quantification
  • 04:36Results: Representative Visualization and Quantification of Cell Division Inhibition
  • 05:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

本文提供了一个分步指南，以研究蛋白质亚细胞定位动力学，并使用高分辨率荧光显微镜监测葡萄球菌和金黄色葡萄球菌的形态变化。

Tags

Live-cell Fluorescence MicroscopySubcellular Protein LocalizationCell Morphology ChangesBacteriaSample PreparationMembrane Staining DyeAgarose SlabHigh-resolution Deconvolution Microscope100x Oil Immersion ObjectiveResolve 3D Imaging SoftwareZ-stacksSample Thickness

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