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活联甘蔗，用于亲和色谱树脂的快速发展 - 抗体捕获作为案例研究

JoVE Journal
Biochimica

This content is Free Access.

JoVE Journal Biochimica

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study

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活联甘蔗，用于亲和色谱树脂的快速发展 - 抗体捕获作为案例研究

DOI:

10.3791/59933-v

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07:53 min

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August 16, 2019

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Matthias Knödler³, Clemens Rühl, Patrick Opdensteinen³, Johannes F. Buyel³

¹Institute for Molecular Biotechnology,RWTH Aachen University, ²Sanofi Deutschland GmbH, ³Fraunhofer Institute for Molecular Biology and Applied Ecology IME,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.

Capitoli

00:04Titolo
01:05Coupling DFE to the Activated Cross-linked Agarose Resin
03:43Testing the Purification of mAbs from Clarified Plants Extracts
05:34Results: Affinity Chromatography Resins Developed by Activated Cross-linked Agarose
07:21Conclusion

Summary

Traduzione automatica

在此过程中，基于DsRed的表位配体被固定，以产生一种高度选择性的亲和树脂，用于从粗植物提取物或细胞培养上生子中捕获单克隆抗体，作为蛋白质A的替代品。

Tags

Activated Cross-linked Agarose Affinity Chromatography Resins Antibody Capture Monoclonal Antibodies Protein A Chromatography Affinity Ligand HIV Neutralizing Antibody 2F5 Dynamic Binding Capacity Resin Reusability Purification Costs NHS-Activated Cross-linked Agarose Coupling Reaction

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