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Genetics

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JoVE Journal Genetics
Identification of Circular RNAs using RNA Sequencing
 

Identification of Circular RNAs using RNA Sequencing

Article DOI: 10.3791/59981-v • 08:25 min • November 14th, 2019
November 14th, 2019 •
1,2, 1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Translational Genomic Research Institute, 2Arizona Alzheimer's Consortium, 3Banner Sun Health Research Institute

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Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.

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Keywords: Circular RNA RNA Sequencing RNase R RNA Cleanup RNA Binding Buffer RNA Wash Buffer Agilent Bioanalyzer TapeStation Total RNA RNA Input Protocol Library Preparation CircRNA Detection Sample Types Functional Role
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