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Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
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JoVE Journal Genetica
Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

DOI:
10.3791/59988-v

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09:12 min

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December 20, 2019

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, , , , , ,
1Laboratory of Biology, BIO@SNS,Scuola Normale Superiore

Capitoli

  • 00:05Titolo
  • 00:32Cell Transfection
  • 01:46Western Blot Sample Extraction
  • 05:00Western Blot
  • 06:51Results: Representative Tau Subcellular Enrichment Analyses
  • 08:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

Tau is a neuronal protein present both in the cytoplasm, where it binds microtubules, and in the nucleus, where it exerts unconventional functions including the modulation of Alzheimer's disease-related genes. Here, we describe a method to investigate the function of nuclear Tau while excluding any interferences coming from cytoplasmic Tau.

Tags

TauSubcellular LocalizationNuclear FunctionSH-SY5YTransfectionTau-NLSTau-NESWestern BlotProtein ExtractionLysis Buffer

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