Rivista
/
Biochimica
/
在氧化应激期间细胞粘附和纤维蛋白扩散动力学
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
Please note that all translations are automatically generated. Click here for the English version.

在氧化应激期间细胞粘附和纤维蛋白扩散动力学

DOI:
10.3791/59989-v

•

10:57 min

•

October 13, 2019

•

, , ,
1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, 2Department of Chemistry,High Point University, 3Biotechnology Program and the Department of Molecular Biomedical Sciences,North Carolina State University

Capitoli

  • 00:04Titolo
  • 01:08Coating Cell Culture Plates with the Extracellular Matrix Protein Fibronectin
  • 02:13Preparing Anchorage-dependent Cells for the Adhesion Assay
  • 05:28Cell Fixation and Antibody Staining for Immunofluorescence
  • 07:41Results: Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
  • 09:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

该方法可用于量化细胞粘附的早期动力学，并将锚固依赖细胞扩散到纤维质。此外，此测定可用于研究改变的氧化还原平衡对细胞扩散和/或细胞粘附相关细胞内信号通路的影响。

Tags

Cellular AdhesionCell SpreadingEpithelial CellsFibronectinOxidative StressExtracellular MatrixCytoskeletal DynamicsRedox StatusMetastatic CancerAnchorage-dependent CellsCell CultureCell LinesOxidantsBSATrypsin-EDTA

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态

生物素化细胞表面蛋白的纯化<emRigicephalus microplus</em>上皮细胞

一种简单的高通量化学筛选方法在秀丽线虫中的应用

蛋白质聚集对酵母细胞氧化应激的影响评价

操纵活细胞构建稳定的3D 蜂窝组件, 无需人工支架

体外支持性脂质双层自组织蛋白模式的重组

用石英谐振器研究磷脂结合蛋白附件 a2 与固体支持的脂质层结合动力学的耗散微重法

一次人气道上皮细胞气道表面液体 pH 值的实时、半自动荧光测量

使用莱迪思光片显微镜可视化表面 T-Cell 受体动力学四维

使用细胞外通量分析仪测量小鼠精子发作期间糖解和氧化磷酸化的变化

Read Article