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一种可定制的酶化生产与纯化天然产物的方法
JoVE Journal
Biochimica
This content is Free Access.
JoVE Journal Biochimica
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
DOI:

07:59 min

October 04, 2019

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Capitoli

  • 00:04Titolo
  • 00:37Diterpenoid Metabolite Production and Transformation in E. coli
  • 02:14Inoculation Culture Preparation
  • 02:59Co-Expression Cultures Preparation and Induction
  • 04:34Metabolite Separation and Purification
  • 05:55Results: Representative Diterpenoid Enzymatic Production and Purification Analyses
  • 07:02Conclusion

Summary

Traduzione automatica

在这里,我们提出了易于使用的协议,通过大肠杆菌或尼科蒂亚纳本塔米亚中的生物合成酶的组合表达来生产和纯化二苯基代谢物,然后是色谱产物净化。由此产生的代谢物适用于各种研究,包括分子结构表征、酶功能研究和生物活性测定。

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