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利用人类诱导多能干细胞生成肿瘤抗原特异性T细胞
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
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利用人类诱导多能干细胞生成肿瘤抗原特异性T细胞

DOI:
10.3791/59997-v

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10:57 min

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October 24, 2019

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, , , , , ,
1Surgery Branch,National Cancer Institute, NIH, 2Center for Cell-Based Therapy,National Cancer Institute, NIH, 3Laboratory of Immunology, Institute for Frontier Life and Medical Sciences,Kyoto University

Capitoli

  • 00:04Titolo
  • 00:58Passaging Human iPSCs on Mouse Embryonic Fibroblasts
  • 02:30Preparation of OP9/DLL1 Cells for Co-culture with hiPSCs
  • 03:51In Vitro Differentiation of hiPSCs into CD8αβ+
  • 08:38Results: hiPSC-derived Hematopoietic Progenitor Cells
  • 09:55Conclusion

Summary

Traduzione automatica

Traduzione automatica

本文介绍了一种利用OP9/DLL1共培养系统生成功能性肿瘤抗原特异性诱导多能干细胞衍生CD8++单阳性T细胞的方法。

Tags

Keywords: Human Induced Pluripotent Stem CellsTumor Antigen-specific T CellsOP9-Delta-1 Co-cultureT Cell GenerationRegenerative MedicineCell-based TherapyNK CellsCell DifferentiationCell PassagingMouse Embryonic FibroblastsOP9 Media

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