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使用核酸探针进行核酸活性的动力学筛选

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

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使用核酸探针进行核酸活性的动力学筛选

DOI:

10.3791/60005-v

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06:52 min

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November 01, 2019

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Alien Balian*^1,2,3, Javier Garcia Gonzalez*^1,2,3, Nora Bastida³, Khadija-Tul Kubra Akhtar³, Baris A. Borsa^2,3, Frank J. Hernandez^2,3

¹Department of Physics, Chemistry and Biology,Linköping University, ²Wallenberg Centre for Molecular Medicine (WCMM), ³Nucleic Acids Technologies Laboratory (NAT-lab),Linköping University

Capitoli

00:05Titolo
00:44Oligonucleotide Library Design and Preparation and Bacteria Culture
02:07Nuclease Activity Assay Setup
03:05Measurement Setup
04:32Data Analysis
05:12Results: Representative Kinetic Screening
06:23Conclusion

Summary

Traduzione automatica

改变的核酸酶活性与不同的人类条件有关，这是其作为生物标志物的潜力的基础。本文提出的模块化且易于实施的筛选方法允许选择特定的核酸探针，以利用核酸酶活性作为疾病的生物标志物。

Tags

Keywords: Nuclease Activity Nucleic Acid Probes Kinetic Screening Biomarker Nuclease Detection Oligonucleotide Library Bacterial Culture Nuclease Assay Fluorometry Salmonella E. Coli

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