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增强的绿色荧光蛋白测定，用于研究原神经元的神经质生长

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

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增强的绿色荧光蛋白测定，用于研究原神经元的神经质生长

DOI:

10.3791/60031-v

•

08:02 min

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October 19, 2019

•

Wai Wa Ray Chan*¹, Wen Li*^1,2, Dik Long Dennis Chau*¹, Kwok-Fai Lau

¹School of Life Sciences,The Chinese University of Hong Kong, Hong Kong Special Administrative Region, ²Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences, Shenzhen

Capitoli

00:04Titolo
00:56Coverslip Preparation
01:36Rat Embryonic Neuron Dissection
02:51Primary Cortical Neuron Culture
04:08Cell Transfection and Fixation
05:16Neurite Overgrowth Measurement
05:58Results: Representative Neurite Growth Modulation
07:29Conclusion

Summary

Traduzione automatica

在本报告中，我们描述了一个简单的方案，通过与EGFP和感兴趣的蛋白质共同转染，研究胚胎大鼠皮质神经元中的神经质外生。

Tags

EGFP Neurite Outgrowth Primary Neurons Transfection Neural Regeneration Brain Trauma Neurodegenerative Diseases Poly-D-Lysine Sprague Dawley Rat Cerebral Hemispheres Cortex Trypsin EDTA Maintenance Medium

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