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Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

DOI:
10.3791/60043-v

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07:25 min

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September 25, 2019

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, , ,
1Department of Microbial Pathogenesis and Immunology,Texas A&M University Health Science Center, 2Department of Ophthalmology,Harvard Medical School

Capitoli

  • 00:04Titolo
  • 00:34Antigen (Ag)‐ Specific CD8+ T cell Generation
  • 02:01Hydrodynamic Herpes B Virus (HBV) Plasmid Delivery for HBV Replication Induction
  • 02:43HBV Replication Reduction
  • 03:38HBV Replication Reduction Analysis
  • 05:30Results: Representative Effects of Stem Cell-Derived Viral Ag-Specific T Lymphocyte HBV Replication Suppression
  • 06:58Conclusion

Summary

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Presented here is a protocol for the effective suppression of hepatitis B virus (HBV) replication in mice by utilizing adoptive cell transfer (ACT) of stem cell-derived viral antigen (Ag)-specific T lymphocytes. This procedure may be adapted for potential ACT-based immunotherapy of HBV infection.

Tags

Keywords: Stem CellViral Antigen-specific T LymphocytesHBV ReplicationIPSC-derived T CellsAdoptive Cell TransferT Cell ReceptorHBV PlasmidHydrodynamic DeliveryCD8-positive T Cells

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