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成年斑马鱼端脑中疟原虫DNA在活体传递的电穿孔方法

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon

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成年斑马鱼端脑中疟原虫DNA在活体传递的电穿孔方法

DOI:

10.3791/60066-v

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06:02 min

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September 13, 2019

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Tamara Durovic², Jovica Ninkovic^3,4

¹Institute of Stem Cell Research,Helmholtz Zentrum München, ²Graduate School of Systemic Neurosciences,Ludwig Maximilian University, ³Department of Cell Biology and Anatomy, Biomedical Center,Ludwig Maximilian University, ⁴Munich Cluster for Systems Neurology (SyNergy)

Capitoli

00:04Titolo
00:50Injection Setup
01:33Plasmid Solution Injection
02:47Electroporation
03:30Results: Representative Ependymoglial Cell Electroporation Outcomes
05:21Conclusion

Summary

Traduzione automatica

这里介绍的是一种在成年斑马鱼端脑中进行质粒DNA传递和脑细胞标记的电穿孔方法。该协议是一种快速而有效的方法，用于可视化和跟踪单个细胞，并开辟了将电穿孔应用于各种基因操作的新的可能性。

Tags

Electroporation Plasmid DNA In Vivo Delivery Zebrafish Telencephalon Ependymoglial Cells Cell Labeling Cell-autonomous Manipulation Cell Biology Cell Delamination Epithelial Homeostasis Cell Division Symmetry Glass Capillary Microinjection Anesthesia Stereomicroscope Microknife Telencephalic Ventricle Ultrasound Gel Electroporation

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