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使用 PRIMO 微模式系统对蛋白质的光诱导分子吸附,研究细胞对细胞外基质蛋白的反应
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins
DOI:

09:49 min

October 11, 2019

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Capitoli

  • 00:04Titolo
  • 00:57Design of Pattern Templates and Plasma Cleaning
  • 01:52Passivation with PLL-PEG
  • 02:54System Calibration
  • 04:36Software Set-up and Photopatterning
  • 05:29Protein Incubation and Blocking Unspecific Binding Sites
  • 06:43Second Round of Photopatterning and Protein Incubation
  • 07:39Results: Micropatterning for the Study of Cell Responses to Extracellular Matrix Proteins
  • 09:03Conclusion

Summary

Traduzione automatica

我们的总体目标是了解细胞如何感知细胞外线索,导致定向的斧头生长。在这里,我们描述了光诱导分子吸附蛋白质的方法,用于生成细胞外基质成分的定义的微模式,以研究控制斧头生长和路径发现的特定事件。

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