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Adsorção molecular induzida por luz de proteínas usando o sistema PRIMO para Micropadronização para estudar as respostas celulares às proteínas da matriz extracelular
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1Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research,The University of Manchester, 2School of Materials,The University of Manchester, 3Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester,Manchester Academic Health Science Centre, 4Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust,Manchester Academic Health Science Centre, 5Department of Pathology,UMC Utrecht
Capitoli
- 00:04Titolo
- 00:57Design of Pattern Templates and Plasma Cleaning
- 01:52Passivation with PLL-PEG
- 02:54System Calibration
- 04:36Software Set-up and Photopatterning
- 05:29Protein Incubation and Blocking Unspecific Binding Sites
- 06:43Second Round of Photopatterning and Protein Incubation
- 07:39Results: Micropatterning for the Study of Cell Responses to Extracellular Matrix Proteins
- 09:03Conclusion
Nosso objetivo geral é entender como as células sentem sinais extracelulares que levam ao crescimento axonal direcionado. Aqui, nós descrevemos a metodologia da adsorção molecular Light-induced das proteínas, usadas para produzir micropatterns definidos de componentes da matriz extracelular a fim estudar os eventos específicos que governam o conseqüência do AXON e o pathfinding.
