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JoVE Journal Cancer Research
A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
 

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

Article DOI: 10.3791/60122-v • 06:27 min • August 8th, 2019
August 8th, 2019 •
1,2, 1
1Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, 2Instituto de Medicina Molecular, Lisbon

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The goal of this protocol is to establish a 3D in vitro model to study the differentiation of cancer-associated fibroblasts (CAFs) in a tumor bulk-like environment, which can be addressed in different analysis systems, such as immunofluorescence, transcriptional analysis and life cell imaging.

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3D Spheroid Model Physiological System Cancer-associated Fibroblasts Differentiation Invasion In Vitro Studies Tumor Tissue Recapitulation 2D System Stiff Plastic Fibroblast Differentiation Cell Matrix Interaction Cell Culture Media MEM FBS Penicillin Streptomycin Methylcellulose Solution Spheroid Formation Solution Normal Fibroblasts Cytokines Integrin Inhibitors TGF Beta One
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