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Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

DOI:
10.3791/60123-v

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06:32 min

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September 04, 2019

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, ,
1College of Osteopathic Medicine,Touro University California

Capitoli

  • 00:04Titolo
  • 00:42Loading A Microinjection Needle with DNA/DOTAP
  • 02:15Microinjecting DNA/DOTAP into Eyebuds of One Day Old Xenopus Embryos
  • 04:30Results: Control and Mutant Optic Axonal Arbors
  • 05:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol aims to demonstrate how to microinject a DNA/DOTAP mixture into eyebuds of one day old Xenopus laevis embryos, and how to image and reconstruct individual green fluorescent protein (GFP) expressing optic axonal arbors in tectal midbrains of intact, living Xenopus tadpoles.

Tags

MicroinjectionDNAXenopus LaevisEmbryosGFPOptic Axonal ArborsTadpolesTransgenesisCell-specificVertebrate ModelGlass MicropipetteMineral OilDOTAPDe-vitellinizationEmbryo Transfer

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