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将DNA微注射到异种胚胎的眼蕾中，以及GFP表达光学轴状轴向轴向的成像，活的Xenopus大白鼠
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
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将DNA微注射到异种胚胎的眼蕾中，以及GFP表达光学轴状轴向轴向的成像，活的Xenopus大白鼠

DOI:
10.3791/60123-v

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06:32 min

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September 04, 2019

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, ,
1College of Osteopathic Medicine,Touro University California

Capitoli

  • 00:04Titolo
  • 00:42Loading A Microinjection Needle with DNA/DOTAP
  • 02:15Microinjecting DNA/DOTAP into Eyebuds of One Day Old Xenopus Embryos
  • 04:30Results: Control and Mutant Optic Axonal Arbors
  • 05:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议旨在演示如何将DNA/DOTAP混合物微注射到一天长的Xenopus laevis胚胎的眼蕾中，以及如何在中脑中图像和重建单个绿色荧光蛋白（GFP），表达光学轴向轴向。完好无损，活着的Xenopus小动物。

Tags

Keywords: MicroinjectionDNAXenopus LaevisEmbryosGFPOptic Axonal ArborsTadpolesTransgenesisCell-specificVertebrate ModelGlass MicropipetteMineral OilDOTAPDe-vitellinizationEmbryo Transfer

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