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Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux
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Biologia
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JoVE Journal Biologia
Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux

Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux

DOI:
10.3791/60133-v

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09:18 min

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September 17, 2019

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, ,
1Division of Pulmonary and Critical Care Medicine,Washington University School of Medicine

Capitoli

  • 00:04Titolo
  • 00:40Protease Inhibitor Administration
  • 01:31Tissue Acquisition and Biochemical Fractionation
  • 05:10Western Blotting
  • 06:48Data Analysis
  • 08:06Results: Representative Experimental Flux Results
  • 08:36Conclusion

Summary

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Traduzione automatica

We describe our protocol for measuring biological rhythms in protein catabolism via autophagy and the proteasome in mouse liver.

Tags

AutophagyProteasomal FluxCircadian RhythmsProtein CatabolismLeupeptinBortezomibHomogenization BufferDounce HomogenizerPost-nuclear SupernatantProtein Concentration

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