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Højeffektiv Transfection af primær makrofager med in vitro transkriberet mRNA
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1Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne,University of Cologne, 2Center for Molecular Medicine Cologne, 3Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), 4German Center for Infection Research (DZIF)
Capitoli
- 00:04Titolo
- 00:43mRNA Generation and Purification
- 02:59Transfection of Macrophages with mRNA
- 04:27Results: Highly Efficient Transfection of Peritoneal Macrophages and Bone Marrow-Derived Macrophages with in vitro Transcribed mRNA
- 06:14Conclusion
Makrofager, især primær makrofager, er udfordrende at transficere, da de specialiserer sig i at opdage molekyler af ikke-selv oprindelse. Vi beskriver en protokol, der giver mulighed for yderst effektiv transfektering af primær makrofager med mRNA genereret fra DNA-skabeloner som plasmider.
