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使用分裂的角质按钮的孔膜内皮器官培养模型

JoVE Journal
Medicina

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JoVE Journal Medicina

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

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使用分裂的角质按钮的孔膜内皮器官培养模型

DOI:

10.3791/60171-v

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08:36 min

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October 06, 2019

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Daniel A. Wenzel, Berenike C. Kunzmann, Nils A. Steinhorst, Martin S. Spitzer, Maximilian Schultheiss

¹Department of Ophthalmology,University Medical Center Hamburg-Eppendorf (UKE), ²University Eye Hospital, Center For Ophthalmology,University Hospital Tübingen

Capitoli

00:04Titolo
00:55Porcine Eye Preparation
01:35Dissection and Incubation
03:43Microscopy and Endothelium Examination
05:11Corneal Endothelium Image Processing
06:15Corneal Endothelial Cell Density and Morphological Parameter Analysis
06:49Results: Representative Corneal Button Culture Analyses
08:12Conclusion

Summary

Traduzione automatica

这里提出了猪分裂角膜纽扣的制备和培养的分步方案。由于这种通常培养的器官培养模型显示细胞死亡率在15天内，相当于人类捐赠角膜，它代表了第一个模型，允许长期种植非人类角膜，而不添加有毒的dextran。

Tags

Porcine Corneal Endothelial Organ Culture Split Corneal Buttons Corneal Endothelial Cell Cultivation Disinfection Fetal Calf Serum Dextran-free Culture Medium Eye Bulb Holder Corneal Trephination Suture Anterior Eye Chamber

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