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Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction

Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction

DOI:
10.3791/60175-v

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06:19 min

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November 06, 2019

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,
1Section of Immunology, Vetsuisse Faculty,University of Zürich

Capitoli

  • 00:04Titolo
  • 01:02Malassezia Inoculum Preparation
  • 02:26Malassezia Infection
  • 03:29Malassezia-Induced Skin Inflammation and Fungal Burden Analysis
  • 04:35Results: Representative Malassezia Skin Infection Analyses
  • 05:23Conclusion

Summary

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This protocol outlines the establishment of a mouse model for studying Malassezia-host interactions in the skin. It describes the cultivation of Malassezia in vitro, the infection of the murine skin with Malassezia, and the subsequent analysis of the inflammation and the fungal burden in the skin tissue.

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Keywords: MalasseziaFungus-host InteractionMouse ModelSkin InfectionInnate ImmunityAdaptive ImmunitySkin DisordersAtopic DermatitisSkin CommensalsImmune ResponseSkin InflammationOD 600Olive Oil SuspensionEar SkinTape StrippingC57 Black6 Mice

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