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感染小鼠与马拉塞齐亚spp.研究真菌-主机相互作用
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Infecting Mice with Malassezia spp. to Study the Fungus-Host Interaction
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感染小鼠与马拉塞齐亚spp.研究真菌-主机相互作用

DOI:
10.3791/60175-v

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06:19 min

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November 06, 2019

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,
1Section of Immunology, Vetsuisse Faculty,University of Zürich

Capitoli

  • 00:04Titolo
  • 01:02Malassezia Inoculum Preparation
  • 02:26Malassezia Infection
  • 03:29Malassezia-Induced Skin Inflammation and Fungal Burden Analysis
  • 04:35Results: Representative Malassezia Skin Infection Analyses
  • 05:23Conclusion

Summary

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该协议概述了用于研究皮肤中马拉塞齐亚宿主相互作用的小鼠模型。它描述了马拉塞齐亚在体外培养，鼠皮感染马拉塞齐亚，以及随后分析皮肤组织中的炎症和真菌负担。

Tags

MalasseziaFungus-host InteractionMouse ModelSkin InfectionInnate ImmunityAdaptive ImmunitySkin DisordersAtopic DermatitisSkin CommensalsImmune ResponseSkin InflammationOD 600Olive Oil SuspensionEar SkinTape StrippingC57 Black6 Mice

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