Rivista
/
Biochimica
/
Quantification of Coenzyme A in Cells and Tissues
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
Quantification of Coenzyme A in Cells and Tissues

Quantification of Coenzyme A in Cells and Tissues

DOI:
10.3791/60182-v

•

08:51 min

•

September 27, 2019

•

, , ,
1Department of Infectious Diseases,St. Jude Children’s Research Hospital

Capitoli

  • 00:05Titolo
  • 01:30Extraction and Derivatization of CoA (Coenzyme A) in Cultured Cells
  • 03:32Extraction & Derivatization of CoA in Tissues
  • 05:31Sample Clean-up with Solid Phase Extraction Column
  • 06:47Results: HPLC Profiles and CoA-bimane Standard Curves
  • 07:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

This method describes sample preparation from cultured cells and animal tissues, extraction and derivatization of coenzyme A in the samples, followed by high pressure liquid chromatography for purification and quantification of the derivatized coenzyme A by absorbance or fluorescence detection.

Tags

Keywords: Coenzyme ACoACellular MetabolismNeurodegenerationBrain Iron AccumulationCoA Biosynthetic PathwaySample PreparationSolid-phase ExtractionCell CulturePotassium HydroxideTrizma-hydrochlorideMBBrThiol

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/60182?article_type=v

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
check_url/it/60182?article_type=v

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
check_url/it/60182?article_type=v

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
check_url/it/60182?article_type=v

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
check_url/it/60182?article_type=v

Quantification of Hypopigmentation Activity In Vitro
check_url/it/60182?article_type=v

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
check_url/it/60182?article_type=v

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
check_url/it/60182?article_type=v

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
check_url/it/60182?article_type=v

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
check_url/it/60182?article_type=v

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Read Article