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使用噬菌体显示肽库筛选和识别针对纤维细胞生长因子受体2的小肽
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JoVE Journal Bioingegneria
Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
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使用噬菌体显示肽库筛选和识别针对纤维细胞生长因子受体2的小肽

DOI:
10.3791/60189-v

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07:32 min

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September 30, 2019

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, , ,
1Institute of Biomedicine & Department of Cell Biology, Jinan University, National Engineering Research Center of Genetic Medicine,Guangdong Provincial Key Laboratory of Bioengineering Medicine

Capitoli

  • 00:04Titolo
  • 00:54First Round of Biopanning: Screening Phage Clones that Bind to the Extracellular Domain of FGFR2
  • 02:26Titer Determination of the Eluted Phages
  • 03:28Phage Amplification
  • 05:38Results: Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2
  • 06:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

在此，我们提出了使用噬菌体显示肽库筛选与 FGFR2 结合的小肽的详细方案。我们进一步分析了所选肽在体外对FGFR2的亲和力及其抑制细胞增殖的能力。

Tags

Phage DisplayPeptide LibraryFGFR2ScreeningIdentificationSmall PeptidesAffinityHigh-throughputBindingAgonistAntagonistAllosteric Modulator

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