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通过心肌毒素损伤和在 Vivo 中注射自传递 siRNA 在骨骼肌再生过程中的卫星细胞功能分析

JoVE Journal
Genetica

This content is Free Access.

JoVE Journal Genetica

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

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通过心肌毒素损伤和在 Vivo 中注射自传递 siRNA 在骨骼肌再生过程中的卫星细胞功能分析

DOI:

10.3791/60194-v

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06:37 min

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September 18, 2019

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Hellen E. Ahrens*¹, Henriette Henze*¹, Svenja C. Schüler, Manuel Schmidt, Sören S. Hüttner, Julia von Maltzahn

¹Leibniz-Institute on Aging – Fritz-Lipmann-Institute

Capitoli

00:04Titolo
00:42Cardiotoxin-induced Muscle Injury and Self-delivering Small Interfering (si)RNA Injection
02:21Tibialis Anterior Muscle Harvest
04:10Results: Representative Skeletal Muscle Injury and Regeneration Analyses
06:02Conclusion

Summary

Traduzione automatica

我们描述了一种体内方法，通过使用心肌心肌的心肌毒素介导损伤和自交付siRNA的注射的组合，研究卫星细胞中特定基因的功能损失。

Tags

Satellite Cell Skeletal Muscle Regeneration Cardiotoxin Injury Self-delivering SiRNA In Vivo Gene Function Muscle Injury

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