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A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones
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Biologia
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JoVE Journal Biologia
A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

DOI:
10.3791/60197-v

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10:11 min

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September 16, 2019

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, , , ,
1Department of Biomedical Research,University of Veterinary Medicine Vienna

Capitoli

  • 00:04Titolo
  • 01:00Animals and Freeze Embedding
  • 01:41Section Preparation
  • 03:26Rapid Staining Protocol
  • 04:57Laser Capture Microdissection
  • 06:27RNA Extraction
  • 07:43Results: Analysis of RNA Obtained from Fresh-Frozen Mouse Bones
  • 09:10Conclusion

Summary

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A laser capture microdissection (LCM) protocol was developed to obtain sufficient quantity of high-quality RNA for gene expression analysis in bone cells. The current study focusses on mouse femur sections. However, the LCM protocol reported here can be used to study gene expression in cells of any hard tissue.

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Laser Capture MicrodissectionRNA ExtractionMouse BoneCryosectionsGene Expression AnalysisOCT CompoundCryostat

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