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Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
JoVE Journal
Medicina
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JoVE Journal Medicina
Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

DOI:
10.3791/60213-v

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08:23 min

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November 05, 2019

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, , , , , ,
1Laboratory of Clinical Chemistry and Hematology, University Medical Center Utrecht,Utrecht University, 2Van Creveldkliniek, University Medical Center Utrecht,Utrecht University, 3Department of Pediatrics, Division of Hematology/Oncology,Baylor College of Medicine

Capitoli

  • 00:04Titolo
  • 01:12Start-up of the Ektacytometer
  • 02:13Sample Collection and Preparation
  • 03:07Oxygen Gradient Ektacytometry Measurement
  • 04:41Cleaning of the Ektacytometer
  • 05:29Shutdown of the Machine
  • 06:02Results: Characterization of Sickling Oxygen Gradient Ektacytometry
  • 07:32Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present oxygen gradient ektacytometry, a rapid and reproducible method to measure red blood cell deformability in samples from patients with sickle cell disease under controlled deoxygenation and reoxygenation. This technique provides a way to study red blood cell sickling and to monitor sickle cell disease treatment efficacy.

Tags

Oxygen Gradient EktacytometrySicklingSickle Cell DiseaseFunctional AssayBiomarkerDisease SeverityTreatment EfficacySample PreparationStandardizationDeoxygenationComplete Blood Count

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