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Avaliando a capacidade de diferenciação das células epitelias da próstata do camundongo usando a cultura organóide
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1Molecular Biology Interdepartmental Program,University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology,University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine,University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research,University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center,University of California, Los Angeles, 6Molecular Biology Institute,University of California, Los Angeles
Capitoli
- 00:05Titolo
- 00:52Plating Sorted Prostate Epithelial Cells into Primary Mouse Organoid Culture
- 03:29Extracting Protein Lysate from Prostate Organoids for Western Blot Analysis
- 04:50Fixing and Staining Prostate Organoids for Immunohistochemical Analysis by Whole-Mount Confocal Microscopy
- 06:44Tissue Clearing and Mounting of the Stained Prostate Organoids for Whole-mount Confocal Microscopy
- 07:57Results: Analysis of Lineage Marker Expression in Prostate Organoids by Western Blot and Whole-Mount Confocal Microscopy
- 09:44Conclusion
Os organóides da próstata do rato representam um contexto prometedor para avaliar os mecanismos que regulam a diferenciação. Este artigo descreve uma abordagem melhorada para estabelecer organóides da próstata e introduz métodos para (1) coletar lysato de proteína de organóides e (2) corrigir e manchar organóides para microscopia confocal de montagem inteira.
