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Evaluación de la capacidad de diferenciación de las células epiteliales de próstata de ratón utilizando el cultivo organoide
This content is Free Access.
1Molecular Biology Interdepartmental Program,University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology,University of California, Los Angeles, 3Department of Urology, David Geffen School of Medicine,University of California, Los Angeles, 4Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research,University of California, Los Angeles, 5Jonsson Comprehensive Cancer Center,University of California, Los Angeles, 6Molecular Biology Institute,University of California, Los Angeles
Capitoli
- 00:05Titolo
- 00:52Plating Sorted Prostate Epithelial Cells into Primary Mouse Organoid Culture
- 03:29Extracting Protein Lysate from Prostate Organoids for Western Blot Analysis
- 04:50Fixing and Staining Prostate Organoids for Immunohistochemical Analysis by Whole-Mount Confocal Microscopy
- 06:44Tissue Clearing and Mounting of the Stained Prostate Organoids for Whole-mount Confocal Microscopy
- 07:57Results: Analysis of Lineage Marker Expression in Prostate Organoids by Western Blot and Whole-Mount Confocal Microscopy
- 09:44Conclusion
Los organoides de próstata de ratón representan un contexto prometedor para evaluar los mecanismos que regulan la diferenciación. Este artículo describe un enfoque mejorado para establecer organoides de próstata, e introduce métodos para (1) recoger el lisato de proteína de los organoides, y (2) fijar y manchar los organoides para la microscopía confocal de montaje completo.
