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在胶原蛋白诱发关节炎模型中，用于处理小鼠爪的冷冻粉碎协议，用于评估组织炎症的分子通路
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
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在胶原蛋白诱发关节炎模型中，用于处理小鼠爪的冷冻粉碎协议，用于评估组织炎症的分子通路

DOI:
10.3791/60229-v

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11:03 min

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October 30, 2019

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, , , , ,
1Immunology,Janssen Research & Development, Inc.

Capitoli

  • 00:04Titolo
  • 01:55Cryogenic Freezer Mill-based Pulverization Method
  • 04:11RNA Extraction
  • 06:36Protein Extraction
  • 07:57Results: Evaluating the Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
  • 09:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

开发了一种使用液氮冷冻机加工鼠爪的低温粉碎方法，以提高从组织中提取的RNA或蛋白质的产量和质量，并分析与炎症相关的分子图谱反应。

Tags

Cryo-pulverizationCollagen-induced Arthritis ModelMolecular ProfilingProteomic AnalysisTranscriptional ProfilingRNA IsolationArthritis ResearchPreclinical ModelFreezer MillLiquid Nitrogen

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