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Celle type-specifik genekspression profilering i muse leveren
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JoVE Journal Biologia
Cell Type-specific Gene Expression Profiling in the Mouse Liver

Celle type-specifik genekspression profilering i muse leveren

DOI:
10.3791/60242-v

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10:06 min

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September 17, 2019

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, ,
1Department of Genetics,University of Pennsylvania, 2Department of Medicine,University of Pennsylvania

Capitoli

  • 00:04Titolo
  • 01:21Conjugation of Antibodies to Magnetic Beads
  • 03:36Liver Tissue Lysis
  • 05:33Immunoprecipitation and RNA Isolation
  • 07:37Results: Cell Type-specific Isolation of High-quality RNA by Trap
  • 09:03Conclusion

Summary

Traduzione automatica

Traduzione automatica

Oversættelse af ribosom affinitets rensning (Trap) muliggør hurtig og effektiv isolering af celletype specifik oversættelse af mRNA. Her demonstrerer vi en metode, der kombinerer Hydrodynamisk hale-vene injektion i en musemodel af leveren genindsættelse og fælde for at undersøge udtryks profilen for at genbefolde hepatocytter.

Tags

TRAP-seqCell Type-specificGene ExpressionLiverHepatocytesLiver InjuryCell TypesMagnetic BeadsProtein LAntibody

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