Rivista
/
Bioingegneria
/
Bulk Droplet Vitrification for Primary Hepatocyte Preservation
JoVE Journal
Bioingegneria
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Bioingegneria
Bulk Droplet Vitrification for Primary Hepatocyte Preservation

Bulk Droplet Vitrification for Primary Hepatocyte Preservation

DOI:
10.3791/60250-v

•

11:07 min

•

October 25, 2019

•

, , , , , , , ,
1Center for Engineering in Medicine,Harvard Medical School, Massachusetts General Hospital, 2Shriners Hospitals for Children, Boston, 3Department of Surgery,University of Amsterdam

Capitoli

  • 00:04Titolo
  • 01:01Vitrification Solutions and Apparatus Preparation
  • 03:47Hepatocyte Pre-incubation with Cryoprotective Agents (CPAs)
  • 04:59Bulk Droplet Vitrification
  • 05:51Cryogenic Storage
  • 07:00Rewarming of the Vitrified Hepatocyte Droplets
  • 09:05Results: Comparison of Bulk Droplet Vitrification to Classic Cryopreservation
  • 10:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

This manuscript describes an ice-free cryopreservation method for large quantities of rat hepatocytes whereby primary cells are pre-incubated with cryoprotective agents at a low concentration and vitrified in large droplets.

Tags

Keywords: Bulk Droplet VitrificationPrimary Hepatocyte PreservationCryoprotective AgentsOsmotic DehydrationCell CryopreservationHepatocyte TransplantationBio-artificial LiverBlood ProductsStem Cells

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/60250?article_type=v

Fluorescence detection methods for microfluidic droplet platforms
check_url/it/60250?article_type=v

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
check_url/it/60250?article_type=v

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
check_url/it/60250?article_type=v

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
check_url/it/60250?article_type=v

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
check_url/it/60250?article_type=v

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
check_url/it/60250?article_type=v

Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
check_url/it/60250?article_type=v

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
check_url/it/60250?article_type=v

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
check_url/it/60250?article_type=v

Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator

Read Article