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Berikelse av Pachytene Spermatocytter og via fra mus testiklene bruke standard laboratorium utstyr
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
DOI:

10:22 min

September 17, 2019

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Capitoli

  • 00:04Titolo
  • 00:58Animal Dissection and Preparation of Germ Cell Suspension
  • 04:50Separation of Germ Cells Through the Discontinuous BSA Gradient
  • 06:02Collection of Enriched Germ Cell Fractions
  • 06:52Analysis of Cell Fractions
  • 08:06Results: MDR Cell Enrichment
  • 09:29Conclusion

Summary

Traduzione automatica

Presentert her er en protokoll for berikende pachytene spermatocytter, runde via, og elongating via fra voksen mus testiklene bruker en usammenhengende storfe serum albumin tetthet gradient med standard laboratorieutstyr.

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