Rivista
/
Ricerca sul cancro
/
Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy
JoVE Journal
Ricerca sul cancro
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Ricerca sul cancro
Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

DOI:
10.3791/60273-v

•

09:17 min

•

September 16, 2019

•

, ,
1School of Applied Sciences,University of Huddersfield, 2Laboratory of Radiotherapy and Experimental Radiation Oncology, Humbertus Wald Tumorzentrum,University Cancer Center Hamburg, University Medical Center Hamburg-Eppendorf

Capitoli

  • 00:04Titolo
  • 01:01Generation of Cell Spheroids
  • 02:15Collagen Invasion Assay
  • 04:15Preparation of Collagen Embedded Spheroids and Migratory Cells for Confocal Microscopy
  • 07:13Results: Cell Migration and Invasion
  • 08:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

The effects of migrastatic inhibitors on glioma cancer cell migration in three-dimensional (3D) invasion assays using a histone deacetylase (HDAC) inhibitor are characterized by high-resolution confocal microscopy.

Tags

3D Tumor SpheroidMigrastatic InhibitorsConfocal MicroscopyRicerca sul cancroCytotoxic DrugsCell MigrationCell Proliferation3D Cell CultureGliomaCollagenCell Invasion

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

A 3D Organotypic Melanoma Spheroid Skin Model

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

Read Article