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微生物相互作用研究的高通量共培养测定
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
High Throughput Co-culture Assays for the Investigation of Microbial Interactions
DOI:

07:00 min

October 15, 2019

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Capitoli

  • 00:04Titolo
  • 01:06Preparation of Overnight Cultures and Bioassay Plates
  • 02:20Inoculating Bioassay Plates with the Test Organism
  • 02:58Preparation and Inoculation of the Target Organism
  • 05:02Results: Co-culture Interaction Assays Uncover Siderophore-mediated Inhibition of Coagulase-negative staphylococci by Corynebacterium propinquum
  • 06:06Conclusion

Summary

Traduzione automatica

该协议中提出的共区域互测定成本低廉、吞吐量高且简单。这些测定可用于观察共培养中的微生物相互作用,识别相互作用模式,并描述感兴趣的微生物菌株对人类和环境病原体的抑制潜力。

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