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使用荧光原位杂交对毛尿胃肠道中的念珠菌的可视化
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Visualization of Candida albicans in the Murine Gastrointestinal Tract Using Fluorescent In Situ Hybridization
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使用荧光原位杂交对毛尿胃肠道中的念珠菌的可视化

DOI:
10.3791/60283-v

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10:08 min

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November 05, 2019

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, ,
1Department of Microbiology and Immunology,University of California, San Francisco, 2Department of Medicine, Division of Infectious Disease,University of California, San Francisco

Capitoli

  • 00:04Titolo
  • 01:28Gastrointestinal Colonization of Mice with C. albicans Using Oral Gavage
  • 03:30Preparation of GI Tissues for Histology
  • 05:50FISH Staining in GI Tract Tissues
  • 08:22Results: FISH-stained C. albicans In Vitro and in Different Gut Compartments
  • 09:17Conclusion

Summary

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该协议的目的是可视化念珠菌的细胞形状和定位在哺乳动物胃肠道。

Tags

Candida AlbicansFluorescent In Situ HybridizationGastrointestinal TractMurineVisualizationMicrobiotaInflammatory Bowel DiseaseGavageDissectionAnimal Model

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