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Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons
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Capitoli
- 00:04Titolo
- 00:54Larval Brain Explant Dissection and Mounting
- 02:04Live Time-Lapse Imaging
- 03:02Drift Correction and Deconvolution
- 03:54Image Annotation
- 05:06Dynamic Dendritic Branch Calculation
- 06:43Results: Representative Dynamic Dendritic Branch Terminal Tracking
- 07:34Conclusion
Here, we describe the method we employed to image highly motile dendritic filopodia in a live preparation of the Drosophila larval brain, and the protocol we developed to quantify time-lapse 3D imaging datasets for quantitative assessments of dendrite dynamics in developing neurons.
