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在伪单体中生成帧内基因删除突变体，并在简单小鼠感染模型中测试病毒衰减
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
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在伪单体中生成帧内基因删除突变体，并在简单小鼠感染模型中测试病毒衰减

DOI:
10.3791/60297-v

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08:34 min

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January 08, 2020

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, ,
1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics,Joan C. Edwards School of Medicine at Marshall University

Capitoli

  • 00:05Titolo
  • 01:24Detection of Double-crossover Recombinants of P. aeruginosa
  • 02:24Confirmation of Gene Deletion with Colony PCR
  • 03:07Visualization of Bioluminescence-labeled Bacteria in Mice
  • 05:42Inoculation of Animals with Bacterial Strains
  • 06:28Results: Evaluation of Attenuated P. aeruginosa Strain
  • 07:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述了一个简单和可重复的小鼠感染模型协议，以评估与美国食品和药物管理局（FDA）批准的大肠杆菌用于商业用途的假单抗病毒的遗传改性菌株的衰减。

Tags

Pseudomonas AeruginosaIn-frame Gene DeletionVirulence AttenuationMouse Infection ModelReproducibilityCrossover RecombinantColony PCRDouble Crossover RecombinantCarbenicillin SensitivitySucrose ResistanceGene Mutation

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