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Experimental Approach to Examine Leptin Signaling in the Carotid Bodies and its Effects on Control of Breathing
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Biologia
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JoVE Journal Biologia
Experimental Approach to Examine Leptin Signaling in the Carotid Bodies and its Effects on Control of Breathing

Experimental Approach to Examine Leptin Signaling in the Carotid Bodies and its Effects on Control of Breathing

DOI:
10.3791/60298-v

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05:45 min

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October 25, 2019

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, , ,
1Department of Medicine,Johns Hopkins University, 2Instituto de Biomedicina de Sevilla (IBiS), Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES),Hospital Universitario Virgen del Rocío/Universidad de Sevilla

Capitoli

  • 00:04Titolo
  • 00:38Hypoxic Ventilatory Response (HVR) Recording
  • 02:47Carotid Sinus Nerve Dissection (CSND)
  • 03:37Induction of Leptin Receptor Long Isoform (LepRb) Expression
  • 04:27Results: Representative Effects of Leptin and LepRb Expression on HVR
  • 05:17Conclusion

Summary

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Our study focuses on the effects of leptin signaling in carotid body (CB) on the hypoxic ventilatory response (HVR). We performed 'loss of function' experiments measuring the effect of leptin on HVR after CB denervation and 'gain of function' experiments measuring HVR after overexpression of the leptin receptor in CB.

Tags

Leptin SignalingCarotid BodiesRespiratory EffectsHypoxiaWhole Body PlethysmographyCarotid Sinus Nerve DenervationAdenovirus TransfectionOxygen SaturationRespiratory Signals

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