Rivista
/
Neuroscienze
/
Cochlear Surface Preparation in the Adult Mouse
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Cochlear Surface Preparation in the Adult Mouse

Cochlear Surface Preparation in the Adult Mouse

DOI:
10.3791/60299-v

•

09:51 min

•

November 06, 2019

•

, , ,
1Department of Pathology and Laboratory Medicine,Medical University of South Carolina, 2MOE Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences,Southeast University

Capitoli

  • 00:05Titolo
  • 01:15Temporal Bone Extraction, Fixation, and Perfusion
  • 02:42Temporal Bone Decalcification
  • 03:31Cochlear Epithelium Microdissection
  • 05:56Cochlear Synapse Immunolabeling
  • 07:52Results: Representative Cochlear Preparation Imaging
  • 09:18Conclusion

Summary

Traduzione automatica

Traduzione automatica

This article presents a modified cochlear surface preparation method that requires decalcification and use of a cell and tissue adhesive to adhere the pieces of cochlear epithelia to 10 mm round cover slips for immunohistochemistry in adult mouse cochleae.

Tags

Cochlear Surface PreparationImmunohistochemistryConfocal ImagingCochlear MicrodissectionCochlear EpitheliumHair Cell RegenerationTemporal Bone ExtractionCochlear FixationEDTA Decalcification

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

Long-term Time Lapse Imaging of Mouse Cochlear Explants

Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea

Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains

Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

Read Article