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1Radboud Institute for Molecular Life Sciences, Department of Pathology,Radboud University Medical Center, 2Radboud Institute for Molecular Life Sciences, Amalia Children’s Hospital, Department of Paediatric Nephrology,Radboud University Medical Center, 3Radboud Institute for Health Sciences, Department of Nephrology,Radboud University Medical Center, 4Radboud Institute for Molecular Life Sciences, Department of Nephrology,Radboud University Medical Center
Capitoli
- 00:04Introduction
- 00:39Mouse Kidney Dissection
- 01:04Isolation of Glomeruli from Mouse Kidney
- 02:25Culturing of Glomerular Outgrowth
- 03:40Analysis of Parietal Epithelial Cell Proliferation
- 04:47Results: Analysis of Glomerular Outgrowth
- 06:08Conclusion
Dieser Artikel beschreibt eine Methode zur Kultivierung und Analyse glomerulären parietalen Epithelzellauswüchsen von verkapselten Glomeruli, die aus der Mausniere isoliert sind. Diese Methode kann verwendet werden, um Wege zu untersuchen, die an der Proliferation und Migration von parietalen Epithelzellen beteiligt sind.
