È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:43Brain Region Dissection
- 01:43Mechanical Tissue Dissociation
- 02:40Myelin Removal
- 04:25Staining for Fluorescence-activated Cell Sorting
- 05:18Index FACS Sorting
- 05:46Single-cell RNA-sequencing Library Preparation
- 07:17Results: Isolation of Region-Specific Microglia for Deep Single-Cell RNA Sequencing
- 09:16Conclusion
