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Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
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Genetica
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JoVE Journal Genetica
Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

DOI:
10.3791/60366-v

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11:54 min

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October 20, 2019

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, , ,
1Department of Experimental Medicine,McGill University, 2Department of Human Genetics,McGill University, 3Department of Obstetrics and Gynecology,McGill University

Capitoli

  • 00:05Titolo
  • 00:32Genotyping
  • 05:23Flow Cytometry
  • 09:30Results: Select Genotyping Results Showing a Triploid Dispermic Genotype
  • 11:02Conclusion

Summary

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Hydatidiform moles are abnormal human pregnancies with heterogeneous aetiologies that can be classified according to their morphological features and parental contribution to the molar genomes. Here, protocols of multiplex microsatellite DNA genotyping and flow cytometry of formalin-fixed paraffin-embedded molar tissues are described in detail, together with results’ interpretation and integration.

Tags

Keywords: Microsatellite DNA GenotypingFlow CytometryPloidy AnalysisFormalin-fixed Paraffin-embeddedContaminationMaternal DNADiagnosisH&E StainingChorionic VilliDNA Extraction

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